Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients

Chen Chumo

doi:doi:10.11648/j.sd.20190702.12

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Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients

Chen Chumo

Published in Science Discovery (Volume 7, Issue 2)

Received: 22 April 2019 Published: 23 May 2019

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Abstract

Colorectal cancer is a kind of malignant tumor, which results from life habits and genetic factors. This research project is called "Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients", which belongs to basic research. Next-generation sequencing technology (NGS), also known as high-throughput sequencing technology, can comprehensively obtain whole genome and transcriptome information of cells and tissues through experimental operations including DNA or RNA extraction, purification, library construction, and bioinformatics analysis; in this way, NGS can screen the mutation and abnormal expression of tumor genes and provide relatively accurate therapeutic targets as the guidance of medication. In this study, the transcriptome of five pairs of tumor tissue samples (t: tumor) and normal tissue samples (n: normal) from five patients with colorectal cancer were compared by NGS: the total RNA from those tissues were extracted to construct mRNA library, from which cancer-related transcriptome information was obtained through NGS-based RNA-seq technology and bioinformatics analysis, which may guide clinical research and treatment. The RNA-seq data of transcriptome information from the colorectal cancer patients reflected variably expressed pattern in the comparison of CRCs/normal tissue pairs; two notable targets, COL1A1 and SPP1, were consistent with two other previous researches and matched pathways enrichment with one of them.

Published in	Science Discovery (Volume 7, Issue 2)
DOI	10.11648/j.sd.20190702.12
Page(s)	65-71
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

NGS RNA-Seq Technology, Transcriptome Comparison, Bioinformatics Analysis, Colorectal Cancer

References

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[16]	Li M., Zhao LM. et al. “Differentially expressed lncRNAs and mRNAs identified by NGS analysis in colorectal cancer patients.” Cancer Med. 2018 Jul 23. doi: 10.1002/cam4.1696.

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Chen Chumo. (2019). Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients. Science Discovery, 7(2), 65-71. https://doi.org/10.11648/j.sd.20190702.12

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Chen Chumo. Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients. Sci. Discov. 2019, 7(2), 65-71. doi: 10.11648/j.sd.20190702.12

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Chen Chumo. Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients. Sci Discov. 2019;7(2):65-71. doi: 10.11648/j.sd.20190702.12

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@article{10.11648/j.sd.20190702.12,
  author = {Chen Chumo},
  title = {Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients},
  journal = {Science Discovery},
  volume = {7},
  number = {2},
  pages = {65-71},
  doi = {10.11648/j.sd.20190702.12},
  url = {https://doi.org/10.11648/j.sd.20190702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20190702.12},
  abstract = {Colorectal cancer is a kind of malignant tumor, which results from life habits and genetic factors. This research project is called "Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients", which belongs to basic research. Next-generation sequencing technology (NGS), also known as high-throughput sequencing technology, can comprehensively obtain whole genome and transcriptome information of cells and tissues through experimental operations including DNA or RNA extraction, purification, library construction, and bioinformatics analysis; in this way, NGS can screen the mutation and abnormal expression of tumor genes and provide relatively accurate therapeutic targets as the guidance of medication. In this study, the transcriptome of five pairs of tumor tissue samples (t: tumor) and normal tissue samples (n: normal) from five patients with colorectal cancer were compared by NGS: the total RNA from those tissues were extracted to construct mRNA library, from which cancer-related transcriptome information was obtained through NGS-based RNA-seq technology and bioinformatics analysis, which may guide clinical research and treatment. The RNA-seq data of transcriptome information from the colorectal cancer patients reflected variably expressed pattern in the comparison of CRCs/normal tissue pairs; two notable targets, COL1A1 and SPP1, were consistent with two other previous researches and matched pathways enrichment with one of them.},
 year = {2019}
}

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TY  - JOUR
T1  - Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients
AU  - Chen Chumo
Y1  - 2019/05/23
PY  - 2019
N1  - https://doi.org/10.11648/j.sd.20190702.12
DO  - 10.11648/j.sd.20190702.12
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 65
EP  - 71
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20190702.12
AB  - Colorectal cancer is a kind of malignant tumor, which results from life habits and genetic factors. This research project is called "Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients", which belongs to basic research. Next-generation sequencing technology (NGS), also known as high-throughput sequencing technology, can comprehensively obtain whole genome and transcriptome information of cells and tissues through experimental operations including DNA or RNA extraction, purification, library construction, and bioinformatics analysis; in this way, NGS can screen the mutation and abnormal expression of tumor genes and provide relatively accurate therapeutic targets as the guidance of medication. In this study, the transcriptome of five pairs of tumor tissue samples (t: tumor) and normal tissue samples (n: normal) from five patients with colorectal cancer were compared by NGS: the total RNA from those tissues were extracted to construct mRNA library, from which cancer-related transcriptome information was obtained through NGS-based RNA-seq technology and bioinformatics analysis, which may guide clinical research and treatment. The RNA-seq data of transcriptome information from the colorectal cancer patients reflected variably expressed pattern in the comparison of CRCs/normal tissue pairs; two notable targets, COL1A1 and SPP1, were consistent with two other previous researches and matched pathways enrichment with one of them.
VL  - 7
IS  - 2
ER  -

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Author Information

Chen Chumo

Shanghai World Foreign Lanuage Academy, Shanghai, China

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Plain Text BibTeX RIS

APA Style

Chen Chumo. (2019). Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients. Science Discovery, 7(2), 65-71. https://doi.org/10.11648/j.sd.20190702.12

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ACS Style

Chen Chumo. Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients. Sci. Discov. 2019, 7(2), 65-71. doi: 10.11648/j.sd.20190702.12

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AMA Style

Chen Chumo. Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients. Sci Discov. 2019;7(2):65-71. doi: 10.11648/j.sd.20190702.12

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@article{10.11648/j.sd.20190702.12,
  author = {Chen Chumo},
  title = {Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients},
  journal = {Science Discovery},
  volume = {7},
  number = {2},
  pages = {65-71},
  doi = {10.11648/j.sd.20190702.12},
  url = {https://doi.org/10.11648/j.sd.20190702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20190702.12},
  abstract = {Colorectal cancer is a kind of malignant tumor, which results from life habits and genetic factors. This research project is called "Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients", which belongs to basic research. Next-generation sequencing technology (NGS), also known as high-throughput sequencing technology, can comprehensively obtain whole genome and transcriptome information of cells and tissues through experimental operations including DNA or RNA extraction, purification, library construction, and bioinformatics analysis; in this way, NGS can screen the mutation and abnormal expression of tumor genes and provide relatively accurate therapeutic targets as the guidance of medication. In this study, the transcriptome of five pairs of tumor tissue samples (t: tumor) and normal tissue samples (n: normal) from five patients with colorectal cancer were compared by NGS: the total RNA from those tissues were extracted to construct mRNA library, from which cancer-related transcriptome information was obtained through NGS-based RNA-seq technology and bioinformatics analysis, which may guide clinical research and treatment. The RNA-seq data of transcriptome information from the colorectal cancer patients reflected variably expressed pattern in the comparison of CRCs/normal tissue pairs; two notable targets, COL1A1 and SPP1, were consistent with two other previous researches and matched pathways enrichment with one of them.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients
AU  - Chen Chumo
Y1  - 2019/05/23
PY  - 2019
N1  - https://doi.org/10.11648/j.sd.20190702.12
DO  - 10.11648/j.sd.20190702.12
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 65
EP  - 71
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20190702.12
AB  - Colorectal cancer is a kind of malignant tumor, which results from life habits and genetic factors. This research project is called "Using NGS Technology for Transcriptome Comparison of Normal and Tumor Tissues in Colorectal Cancer Patients", which belongs to basic research. Next-generation sequencing technology (NGS), also known as high-throughput sequencing technology, can comprehensively obtain whole genome and transcriptome information of cells and tissues through experimental operations including DNA or RNA extraction, purification, library construction, and bioinformatics analysis; in this way, NGS can screen the mutation and abnormal expression of tumor genes and provide relatively accurate therapeutic targets as the guidance of medication. In this study, the transcriptome of five pairs of tumor tissue samples (t: tumor) and normal tissue samples (n: normal) from five patients with colorectal cancer were compared by NGS: the total RNA from those tissues were extracted to construct mRNA library, from which cancer-related transcriptome information was obtained through NGS-based RNA-seq technology and bioinformatics analysis, which may guide clinical research and treatment. The RNA-seq data of transcriptome information from the colorectal cancer patients reflected variably expressed pattern in the comparison of CRCs/normal tissue pairs; two notable targets, COL1A1 and SPP1, were consistent with two other previous researches and matched pathways enrichment with one of them.
VL  - 7
IS  - 2
ER  -

Copy | Download